Abstract 10894: Adipose-derived Stem Cell Sheets Created by a Novel Magnetite Tissue Engineering Method for Myocardial Infarction
Background: Adipose-derived stem cells (ADSCs) are promising source of autologous stem cells for regeneration and/or repair of infracted heart. Here, we investigated a therapeutic potential of ADSC sheets created by a novel magnetite tissue engineering technology (The Mag-TE) for myocardial infarction in an animal model.
Methods and Results: Subcutaneous adipose tissue was obtained from wild-type (WT) mice and ADSCs were isolated using standard methods. ADSCs were cultured and labeled with magnetic nanoparticle-containing liposomes (MCLs). Then MCL-labeled ADSCs were mixed with diluted extracellular matrix (ECM) precursor, and culture dishes were placed on a magnet. Magnetized ADSCs formed multilayered cell sheets after a 24h incubation period. Mice were subjected to myocardial infarction by a permanent ligation of the left anterior descending coronary artery. We then transplanted the ADSC sheet or cell-free collagen gel sheet as a control onto the scared myocardium using the Alnico magnet before skin closure. Cardiac parameters were measured by echocardiogram and angiogenesiswas determined by capillary density stained with anti-CD31 Mab. ADSC sheet-treated mice showed a significant improvement in systolic function, infarct wall thinning and fibrotic length at 14 days after myocardial infarction compared with control mice (P<0.05). Implantation of ADSC sheet also promoted angiogenesis in both infarct area and border zone in WT mice after myocardial infarction (P<0.05). The angiogenic effects of ADSC sheets were attributable to increased expressions of VEGF, bFGF and HGF mRNA in ischemic hearts.
Conclusions: ADSC sheet, created by the Mag-TE system and the ECM precursor embedding system, was successfully engrafted into ischemic heart tissues of WT mice. Implantation of ADSC sheets protects the heart against pathological cardiac remodeling through its ability to promote angiogenesis. ADSC sheets may become a novel regenerative medicine strategy for ischemic heart disease in future.
- © 2012 by American Heart Association, Inc.