Abstract 10893: Development and Remodeling of Newly Formed Blood Vessels in the Specific Hypoxyprobe-positive Area of Infarcted Heart
Background: Reperfusion therapy by catheter intervention is one of the most effective therapies in myocardial infarction. Recanalization can rescue the damaged myocardium in the border zone. However, the border zone of ischemia has not been defined. Elucidation of phenomena of angiogenesis and apoptosis in this area is indispensable to evaluate or develop novel therapeutic modality such as gene therapy and stem cell therapy.
Objective: To clarify the phenomena of angiogenesis and apoptosis in the hypoxic area of infracted mouse heart.
Methods: hypoxyprobe-1 was applied to infracted heart to identify hypoxic area, together with lectin for staining of perfused vessels. Staining of hypoxyprobe-1, lectin and TUNEL was conducted using MI 2, 3, 6, 12, 24 hour, 2, 3, 7 and 14 day samples.
Results: Hypoxic area positively stained with hypoxyprobe-1 was clearly identified in MI 2, 3 and 6 hour samples. Positive area was gradually decreased and limited to the area close to non-ischemic zone. On the other hand, TUNEL positive area time-dependently enlarged from center to border of non-perfused area. Survived myocardium stained with hypoxyprobe-1 in hypoxic area was successfully identified by this staining method (Figure 1). Newly formed vessels in hypoxyprobe positive area began to be formed at day 3 and developed day 3 through day 7 (Figure 2). Moreover, vessel network newly formed by day 7 was remodeled from day7 through day 14.
Conclusions: Border zone of schemic heart was observed spatially and temporally using hypoxyprobe, lectin and TUNEL staining. Newly formed vessels in hypoxic area develop and are remodeled dramatically from day 3 through day 14.
- © 2012 by American Heart Association, Inc.