Abstract 10789: Plasma Free Hydrogen Sulfide Levels are Elevated in Vascular Disease
Background: Peripheral artery disease (PAD) currently afflicts millions of adults in the US with many individuals who are asymptomatic and remain undiagnosed. Only about a third of these patients receive recommended therapies for secondary prevention. A plasma biomarker of vascular disease burden would have a significant public health impact in the identification and care of patients with PAD. We hypothesized that the plasma levels of the gasotransmitter hydrogen sulfide (H2S), known to regulate vascular function, might be altered in patients with and without PAD.
Methods: In this prospective trial, we enrolled 193 patients over the age of 40 presenting for cardiac catheterization or peripheral angiography. In all patients, the ankle brachial index was measured and plasma obtained from existing arterial access for HPLC analytical measurement of plasma free H2S, total nitric oxide levels, plasma nitrite, thrombospondin-1 (TSP-1), IL-6 and soluble ICAMs.
Results: We found that plasma free H2S levels in patients without PAD or CAD were significantly lower when compared to patients with PAD, those with PAD without CAD and those with any vascular disease (see table). Utilizing multiple regression analyses, we found that the elevated H2S levels were independent of the presence of DM, HTN, dyslipidemia, smoking status, race, gender or age. TSP-1, nitrite and total nitric oxide however were not influenced by the presence of PAD, though TSP-1 negatively correlated with total nitric oxide levels. Interestingly, African American males (n=49), had significantly lower free plasma H2S compared to Caucasian males (n=51), 382.41 vs. 474.67 nM (p=0.02).
Conclusion: Free plasma H2S level is significantly increased in patients with vascular disease and may be a useful biomarker to identify individuals at risk for PAD.
- © 2012 by American Heart Association, Inc.