Abstract 10637: Effects of Pedicle Omentum Flap Combined With Cell Sheet Implantation on Vessel Stability, Myocardial Perfusion, and Left Ventricular Reverse Remodeling in Rat Myocardial Infarction Model
Objective: Cell sheet implantation has been shown to induce therapeutic angiogenesis in damaged myocardium, though instability of newly formed vessels may limit the effects. Accelerating maturation of new vessels to generate a well-organized vasculature may thus be crucial for producing therapeutic effects with this treatment. We speculated that a pedicle omentum flap combined with cell sheet therapy may modulate neovascular maturation, leading to enhanced functional recovery.
Methods: Cell sheets were constructed by culturing skeletal myoblasts of GFP-transgenic Lewis rats. Left coronary artery-ligated wild-type Lewis rats were divided into 4 groups; cell sheet transplantation (1.0×107 cells) plus omentum flap (combined), cell sheet alone (sheet), omentum alone (omentum), and sham operation (control) (n=15 for each).
Results: At 12 weeks after treatment, the combined group showed greater numbers of pericyte-lined neovascular endothelium (NG-2+/ILB-4+) and functional endothelium (eNOS+) cells in the peri-infarct myocardium as compared to the other groups (Figure). The combined also showed greater myocardial perfusion in the peri-infarct area, confirmed by PET-MRI. Serial echocardiography indicated persistent improvement in LV function (reverse remodeling) in the combined group (ejection fraction: 36±3 at baseline vs. 47±3 at 4 weeks vs. 51±4% at 12 weeks), but not in the other groups (sheet; 36±4 vs. 44±4 vs. 41±2%, omentum; 37±4 vs. 39±4 vs. 39±3%, control; 38±5 vs. 36±3 vs. 34±3%, respectively). Celiac artery angiography using india ink demonstrated communication between the pedicle omentum and native coronary arteries, which was also shown by histological analysis.
Conclusions: A pedicle omentum combined with cell sheet transplantation increased a well-organized and functional vasculature accompanied with a potent functional recovery in rat myocardial infarction model, suggesting a promising clinical application for failing heart.
- © 2012 by American Heart Association, Inc.