Abstract 9902: Food Restriction Induces Cardiac BDNF/TrkB Signaling and Improves Physical Activity in Obese Mice
Introduction-Brain-derived neurotrophic factor (BDNF) gene is not only expressed in neuron but cardiac muscle and modulate cardiac function. We hypothesized that cardiac expression of BDNF can be induced by food restriction in obesity and that induced BDNF signaling can be beneficial in cardiac dysfunction.
Methods-Obese KKAy mice were ad libitum as a control (CF) or 60% restriction of that eaten by ad libitum (RF). Mice were sacrificed on day 0, 7, 28, and 84 after food restriction.
Results-Body weight and liver weight / body weight ratio in RF were significantly lower than that in CF on days 7, 28 and 84 (P<0.05) but heart weight/ body weight ratio were not changed between mice with CF and RF. Comparative expression of cardiac BDNF mRNA in RF was significantly higher than that in CF (1.2+0.3 vs. 0.8+0.2, P<0.05). Cardiac expression of TrkB in RF was significantly increased than that in CF on day 84. Cardiac expression of brain natriuretic peptide was significantly lower in mice with FR compared with those with CF. Hippocampal expressions of BDNF in RF and were significantly increased compared with those in CF on days 28 and 84. Hippocampal expression of TrkB in RF was significantly higher than in CF on days 28 and 84. Hippocampal weight / body weight ratio was also significantly higher in mice with RF compared with those with CF on days 28 and 84. Daily activity in RF was significantly increased compared with that in FF on day 28 (P<0.05).
Conclusions-Food restriction can induce cardiac BDNF/TrkB signaling with the increase of daily activity in obese mice. Cardiac BDNF plays a role in recovery from obesity. These data indicate that induced expression of BDNF/TrkB signaling can improve cardiac dysfunction in obese mice with the combination of induced expression of hippocampal BDNF/TrkB.
- © 2011 by American Heart Association, Inc.