Abstract 9362: Argon Reduces Neurohistopathological Damage and Preserves Functional Recovery After Cardiac Arrest in Rats
Introduction: Xenon has profound neuroprotective effects in various models of neurological injury and is currently undergoing phase two clinical trials in cardiac arrest patients. However, xenon is very costly which might preclude a widespread use. We hypothesized that the more available Argon might also protect central nervous tissues and functional recovery in a rodent model of global cerebral ischemia.
Material and Methods: 14 male Sprague-Dawley rats were subjected to 7 mins of cardiac arrest and 3 mins of cardiopulmonary resuscitation (CPR). One hour after successful CPR, animals were randomized to ventilation with 70% Argon for 1h in comparison to untreated controls receiving 70% Nitrogen. A neurologic deficit score was calculated on seven days following the experiment before the animals were killed and the brains harvested for histopathological analyses.
Results: All animals survived. Untreated controls exhibited severe neurological dysfunction while animals from the Argon group showed significant improvements at all points in time (Fig. 1). This was paralleled by a significant reduction in the degree of necrotic cell damage in the neocortex and the hippocampal CA 3/4 region (Fig. 2).
Conlusions: Our study demonstrates that a single one hour application of 70% Argon provided a significant reduction in histopathological damage of the neocortex and hippocampus which was associated with a marked improvement in functional neurological recovery.
- © 2011 by American Heart Association, Inc.