Abstract 8525: Adipose-Derived Mesenchymal Stem Cell Protects Kidneys Against Ischemia-Reperfusion Injury Through Suppressing Oxidative Stress and Inflammatory Reaction
Background: Reactive oxygen species are important mediators exerting toxic effects on various organs during ischemia-reperfusion (IR) injury. We hypothesized that adipose-derived mesenchymal stem cells (ADMSCs) protect the kidney against oxidative stress and inflammatory stimuli in rat during renal IR injury.
Methods: Adult male Sprague-Dawley (SD) rats (n=24) were equally randomized into group 1 (sham control), group 2 (IR plus culture medium only), and group 3 (IR plus immediate intra-renal administration of 1.0 × 106 autologous ADMSCs, followed by intravenous ADMSCs at 6h and 24h after IR). The duration of ischemia was 1h, followed by 72 hours of reperfusion before the animals were sacrificed.
Results: Serum creatinine and blood urea-nitrogen levels and the degree of histological abnormalities were markedly lower in group 3 than in group 2 (all p<0.03). The mRNA expressions of inflammatory, oxidative stress, and apoptotic biomarkers were lower, whereas the anti-inflammatory, anti-oxidative, and anti-apoptotic biomarkers were higher in group 3 than in group 2 (all p<0.03). Immunofluorescent staining showed a higher number of CD31+, von Willebrand Factor+, and heme oxygenase (HO)-1+ cells in group 3 than in group 2 (all p<0.05). Western blot showed notably higher NAD(P)H quinone oxidoreductase 1 and HO-1 activities, two indicators of anti-oxidative capacity, in group 3 than those in group 2 (all p<0.04). Immunohistochemical staining showed higher glutathione peroxidase and glutathione reductase activities in group 3 than in group 2 (all p<0.02).
Conclusion: ADMSC therapy minimized kidney damage after IR injury through suppressing oxidative stress and inflammatory response (The possible mechanisms of ADMSC therapy in protecting kidney from IR injury are schematically presented in the following figure).
- © 2011 by American Heart Association, Inc.