Abstract 8422: Cardiac Protein Abnormalities of Human Left Ventricular Tissue in Ischemic and Dilated Cardiomyopathy
Introduction: The development of heart failure (HF) is characterized by progressive alteration of left ventricle structure and function. A proteomic approach could lead to the detection of variations in protein expression of left ventricular tissue from patients with ischemic (ICM) and dilated cardiomyopathy (DCM).
Methods: 24 explanted human hearts, 12 from patients with ICM and 12 with DCM undergoing cardiac transplantation and 6 non-diseased donor hearts (CNT) were analyzed by 2DE. Proteins of interest were identified by mass spectrometry and validated by Western blotting and immunofluorescence.
Results: We encountered 35 differentially regulated spots in the comparison CNT vs. ICM, and 33 in CNT vs. DCM. We identified G3P up-regulation in both ICM (2.53-fold, p<0.0001) and DCM (2.89-fold, p<0.0001). HSP71 was up-regulated only in ICM (3.31-fold, p<0.0001). G3P was related with ventricular function parameters (left ventricular end-systolic diameter (p=0.001), end-diastolic diameter (p=0.001)) in our HF patients. HSP71 was also related with these functional parameters (p<0.05).
Conclusions: We have shown by proteomics analysis that HF is associated with changes in proteins involved in the cellular stress response, respiratory chain and cardiac metabolism. Although we found altered expression of proteins common to both ischemic and dilated etiology, we also found different proteins altered in both groups. In addition, for the first time left ventricular function was related with changes in G3P and HSP71.
- © 2011 by American Heart Association, Inc.