Abstract 283: Efficacy of a Novel Artificial Oxygen Carrier (LHb) on Ameliorating Lethal Arrhythmogenetic Property in Hemorrhagic Shock Heart: Optical Mapping Analysis
Irreversible heart dysfunction despites restoring normal systemic circulation in shock, that is so called “Shock Syndrome”, is caused by prolonged low mean systemic blood pressure (BP) < 40 mmHg, and this heart dysfunction frequently associates with lethal arrhythmias leading to adverse prognosis in the hemorrhagic shock patients. To investigate if a novel artificial oxygen carrier (LHb) is effective to improve arrhythmogenetic property in hemorrhagic shock heart, we performed optical mapping analysis (OM) and electrophysiological study (EPS) in rat's heart obtained from 30% hemorrhagic shock model with repetitive bleedings, where sustained lower mean BP < 40 mmHg was kept for more than 30 min. Hemorrhagic shock was induced in Sprague-Dawley rats (n=32). Rats were resuscitated by LHb, washed red bold cell (wRBC), normal saline (NS) and 5% albumin (5%alb). Hearts were excised, perfused with Tyrode solution and stained with fluorescent dye. Ventricular fibrillation (VF) was induced by burst pacing stimulus to multiple sites in the left ventricle (LV) and OM was performed. VF was induced in all rats resuscitated by NS and 5%alb and their OM showed conduction delay and impaired action potential duration (APD) dispersion (APDd), defined as APDd = maximum APD - minimum APD, in LV. However, all rats rescued by LHb and wRBC had normal OM findings and no VF was induced. Conclusions; LHb can improve deteriorated electrophysiological property in LV of hemorrhagic shock heart and prevent lethal arrhythmias compatible with wRBC. LHb possibly and comparably ameliorates prognosis in massive hemorrhagic shock patients through reducing shock-related lethal arrhythmias with wRBC.
- Shock, cardiogenic
- Ventricular arrhythmia
- Assisted circulation
- Artificial heart/Cardiac support devices
- © 2011 by American Heart Association, Inc.