Abstract 192: Double Doses of Tea Polyphenols Are as Effective as a Single Dose in an Asphyxial Rat Model
Background: Cerebral ischemia/reperfusion injury following cardiac arrest and cardiopulmonary resuscitation (CPR) has been demonstrated. Oxidant injury plays a critical role in the process. Since tea polyphenols from green tea are efficient free radical and singlet oxygen scavengers, and we have demonstrated single dose of tea polyphenols improved the outcome of CPR in an asphyxia rat model. Theoretically, more tea polyhenols will be more efficient. Therefore, we hypothesized that double doses of tea polyphenols will better improve the outcome of CPR via counteracted the oxidant injury than single dose of that.
Methods: Male Sprague-Dawley rats, weighing 200-400 g, were induced cardiac arrest by clamping the trachyeal tubes. At the end of 8 min of clamping, mechanical chest compression at a rate of 180/min was performed. Ventilation was started with room air at 70 breaths per min and tidal volume adjusted to 6 ml. Epinephrine was administered at 1 min of CPR. Animals were randomly divided into three groups: Sal-gro, TP-gro1 and TP-gro2 (treated with either saline, 10mg/kg tea polyphenols or 10mg/kg tea polyphenols following ROSC and with saline, saline, or 10mg/kg tea polyphenols after one hour of ROSC).
Results: There were no significant differences in regard to hemodynamics among three groups before asphyxia. Time from the initiation of asphyxia to cardiac arrest and the duration of CPR were not significant difference among three groups. However, neurological deficit score were better in TP-gro1 and TP-gro2 than that in Sal-gro (P<0.05). And survival time were longer in TP-gro1 and TP-gro2 than that in Sal-gro (P<0.05). There were no significant about neurological deficit score and survival time between TP-gro1 and TP-gro2.
Conclusion: Double doses of tea polyphenols were as effective as single dose of that in a rat of asphyxia cardiac arrest model.
- © 2011 by American Heart Association, Inc.