Abstract 18372: Clinical Threshold of Adenovirus Genome Load in Myocardial Tissues of Patients with Myocarditis and Dilated Cardiomyopathy
Background: Viral myocarditis is rarely recognized hence it is one of the most challenging diagnosis in cardiology. It leads to chronic dilated cardiomyopathy (DCM) and heart failure. Adenovirus is one of the most common cardiotropic viruses that cause viral myocarditis. However, the knowledge on AdV load in adenovirus associated myocarditis or DCM is unknown so far.
Aim: To gain insight into the pathogenesis of AdV associated myocarditis, present study was aimed to determine AdV load in myocardial autopsy tissue of patients with myocarditis or DCM.
Methods and Results: AdV load was determined using Taqman Real Time PCR in myocardial autopsy tissues obtained from 24 (11 male and 13 female) patients with histologically proven myocarditis or DCM whereas 12 noninflamed normal hearts obtained from autopsies served as controls. The AdV associated myocarditis was characterized by high mean viral load of 2034 genome equivalents (ge) per microgram of isolated nucleic acids in DCM patients with myocarditis as compared to viral load of 362 genome equivalents (ge) per microgram in normal hearts (p<0.005). Thus, from this data possibly AdV loads of more than 450 ge per microgram in myocardial autopsy are clinically relevant threshold for the maintenance of myocardial inflammation.
Conclusion: The present study could derive the significant level of adenovirus load of 450 ge per microgram in myocardial tissues to be associated with adenovirus myocarditis and DCM. The knowledge on the clinical threshold of adenovirus load would help in diagnosing the clinically suspected viral myocarditis or DCM cases as well as would help in establishing an antiviral strategy to treat these patients.
- © 2011 by American Heart Association, Inc.