Abstract 17708: Hydrogel Loaded with Mesenchymal Stem Cells Preserves Cardiac Function and Ventricular Remodeling After Myocardial Infarction
Purpose: We hypothesized that the injection of mesenchymal stem cells (MSC) within an hydrogel would improve efficacy of cell therapy after myocardial infarction (MI). We have developed an hydrogel composed to Hydroxypropyl methylcellulose Silanyzed (Si-HPMC).
Methods: The cytocompatibility was evaluated by the MSC viability cultivated in 3D within the hydrogel using cytotoxicity assays. Immediately after coronary ligation, hydrogel, 3.106 MSCs alone or with the hydrogel (MSC+hydrogel) were injected into the myocardium of rats (PBS used as control). Cardiac function was assessed using echocardiography over 56 days and left ventricular (LV) remodeling was assessed using histopathology.
Results: The hydrogel maintained the MSC viability during the whole culture period. In the PBS group, MI induced a reduction in left ventricular ejection fraction (LVEF) (87.4±1.5% at baseline vs 47.4±2.4% at 56 days; p<0.001) and an increase in LV diameters during the whole study. The injection of MSC+hydrogel in acute phase of MI induced an significant increased of LVEF at day 7 up to day 56 as compared to PBS group (at day 56: 47.4±2.4% in PBS group vs 68.5±2.0% in MSC+hydrogel group; p<0.001) and an increase as compared to day 1 after MI (61.2±2.9% at day 1 vs 76.4±1.5 % at day 28; p<0.001 as compared to day 1). The injection of hydrogel alone induced an increase in LVEF at 28 days as compared to PBS group but not at day 7 and 56 and the injection of MSC induced an increase at 28 and 56 days as compared to PBS group, but not at day 7. In the both groups the LV diameters and LVEF are not significantly altered during the whole study as compared to day 1. Interestingly when animals have been treated with MSC+hydrogel, their LVEF was higher at day 7 as compared to EF in MSC group and at day 56 in compared to hydrogel group.
Conclusion: These results showed the major effects of Si-HPMC hydrogel are observed at the short-term. In acute phase of MI, the MSC delivery with hydrogel allowed a better preservation of cardiac function and LV remodeling as compared to injection of MSC alone.
- Tissue engineering
- Cardiac regeneration
- Regenerative medicine stem cells
- Myocardial infarction
- © 2011 by American Heart Association, Inc.