Abstract 16248: Exercise-Induced Mobilization of Endothelial Progenitor Cells Correlates With Endothelial Dysfunction in Patients With Early Manifestation of Coronary Heart Disease
The aim of this study was to assess the mobilization of endothelial progenitor cells (EPCs) induced by physical exercise and to evaluate the correlation of circulating EPCs with endothelial vasomotor function (flow-mediated dilatation, FMD) and carotid intima-media thickness (IMT) in patients with early manifestation of coronary atherosclerosis.
Methods: 60 patients (72% men) with stable CAD (mean age 43 years) who had myocardial infarction <40 years of age were enrolled. EPC defined as CD34+CD133+VEGFR2+ cells were counted by means of FACS. The number of EPCs was measured at rest (EPC baseline), 15 minutes (EPC 15) and 60 minutes (EPC 60) after submaximal treadmill exercise test. Levels of hsCRP, von Willebrand factor, IL-18, HGF and SCF were measured by ELISA. Intima/media thickness (IMT) and brachial artery FMD examination were performed at rest.
Results: In healthy controls there was a significant mobilization of EPCs 15 minutes after the stress test. In patients with early CAD the mobilization was significantly reduced (Table). Plasma levels of vWf, IL-18 and HGF were significantly higher (123 vs 89%, p=0,0002; 71 vs 48 pg/ml; p=0,00003; 225 vs 156 pg/ml,p=0,0002, respectively), while SCF levels (490 vs 1246 pg/ml, p=0,02), as well as FMD (2,4 vs 6,9 %; p<0,00001) were significantly lower in the study group. Levels of hsCRP and vWf correlated negatively with early EPC mobilization (R=-0,35, p=0,006) and carotid IMT correlated negatively with late EPC mobilization after exercise (R=-0,2856, p<0,05).
Conclusions: Treadmill exercise causes significant increase in circulating EPCs, however in patients with early-onset CAD the mobilization if reduced. The number of circulating EPCs shows significant negative correlation with carotid IMT and positive with impaired endothelial function assessed by FMD. Concentration of vWf is higher in patients with early atherosclerosis and correlates negatively with number of EPCs number at rest and after exercise.
- © 2011 by American Heart Association, Inc.