Abstract 15184: MicroRNA Expression Profiling in Circulating Microparticles of Patients with Acute Coronary Sindrome
AIMS: microRNA (miRNA) are small non-coding RNA regulating gene expression on the post-transcriptional level by binding to the target mRNA. Recent studies have shown that circulating microparticles (MP) could carry miRNA delivering them to a target cell. We sought to characterize miRNA expression in MP in ACS patients.
METHODS AND RESULTS: Peripheral venous blood samples were drawn from 20 subjects:10 with Non-ST Elevation Myocardial Infarction (NSTEMI) and 10 controls without angiographic evidence of coronary artery disease. Total RNA was isolated from circulating MP with Trizol based method and pooled. TaqMan microRNA assays were used for miRNA detection (Applied Biosystem). The presence of 14 amplifiable microRNAs, known to be involved in cardiovascular disease, was investigated. In NSTEMI patients mir-206, mir-21 and mir-29b were from 20 to 35 fold higher than controls. mir-1, mir-29a and mir-29c were from 50 to 135 fold higher in NSTEMI than controls (Figure 1).
CONCLUSION: Our data show that miRNA are highly expressed in MP of ACS patients than controls. In particularly, it was observed an up-regulation of mir-1, mir-21, mir-29a, mir-29b, mir-29c and mir-206, known to be related to ischemia-reperfusion injury, apoptosis and myocardial remodelling, thus suggesting a new potential pathogenetic role of MP in atherosclerotic plaque instability and myocardial damage. These findings may also open the avenue to novel therapeutical application of MIR.
- © 2011 by American Heart Association, Inc.