Abstract 14102: Successful Aortic Valve Implantation Using an In-Body Tissue-Engineered, Completely Autologous Valved Conduit (BIOVALVE) in a Goat Model
Purpose: Autologous valved conduits without any artificial materials (Biovalves) were developed, using simple, safe, and economical in-body tissue engineering. In this study, the potential of the Biovalve as an aortic valve was investigated in a goat model.
Methods: Biovalves were prepared by 2-month embedding of the molds, assembled using 2 types of plastic rods, in the subcutaneous spaces of goats. The formation process was continuously watched over by direct observation with wireless video capsule endoscope. After removing the molds, Biovalves (type VI) with 3 leaflets and sinus of Valsalva similar to those of native aortic valves were constituted from perfectly autologous connective tissues. The Biovalves (n=3) were implanted as aortic valves using the apico-aortic bypass method for 2 months.
Results: Postoperative echocardiography and angiography showed smooth movement of the leaflets with little regurgitation under systemic circulation. Histological examination after 2 months showed that α-SMA-positive cells appeared significantly with rich angiogenesis in the conduit and expanded toward the tip of the leaflet. At the sinus portions, marked elastic fibers were formed. The luminal surface was covered with thin pseudointima.
Conclusion: Completely autologous Biovalves with robust and elastic characteristics satisfied the higher requirements of systemic circulation in goats for 2 months with the potential for valvular tissue regeneration.
- © 2011 by American Heart Association, Inc.