Abstract 13728: New Approach for Intracardiac Hemodynamic Measurements: -Echo-Guided Percutaneous Apical Puncture
Objectives: To develop a new technique for measuring intracardiac hemodynamics.
Background: Invasive measurements of intracardiac hemodynamics in animal models have allowed important advances in the understanding of cardiac disease. Currently this is performed either through a carotid arteriotomy or via a thoracotomy and apical insertion. Both these techniques have disadvantages and are not conducive to repeat measurements.
Methods: In thirteen male rats hemodynamic measurements were performed using a new echocardiography-guided percutaneous apical technique. An intravenous catheter was percutaneously inserted into the left ventricle (LV) in the direction of the LV long axis. Through this catheter a micro manometer-tipped pressure catheter was inserted and invasive hemodynamic traces were recorded. After LV recordings, the pressure catheter was advanced into aorta where pressures were obtained. In eleven rats measurements were repeated after one week (n=2), two weeks (n=4), three weeks (n=4) or four weeks (n=1). The measurements of the percutaneous technique were compared to a carotid arteriotomy approach.
Results: Of thirteen rats subjected to the procedure the survival rate was 85%. Of eleven rats that had procedure repeated three rats died (73%). In three rats invasive measurements were performed using a carotid arteriotomy prior to the percutaneous technique. The mean difference was 10mmHg±4mmHg for LV end systolic pressure and 1mmHg±1mmHg for LV end diastolic pressures. The mean procedure time was 21±3min and 6±1min for carotid and percutaneous technique, respectively.
Conclusion: We have successfully developed a percutaneous technique for insertion of left ventricular micro-tip catheters in rats.
- © 2011 by American Heart Association, Inc.