Abstract 13423: Pm10-Induced Nadph Oxidase Activation: A New Link Between Air Pollution and Oxidative Stress
Background: Ambient and occupational exposure to Particulate Matter (PM) has been linked with increased hospitalization and mortality from cardiovascular disease, respiratory disease, and lung cancer. Extensive evidence indicates that generation of oxidative stress contributes to the health effects associated with ambient and occupational PM exposure. Aim of the present study is to investigate whether PM exposure determine changes of oxidative stress markers in workers of a steel plant with well-characterized exposure to air particles.
Methods and Results: We recruited 113 male healthy workers (mean age 42 years) who had been working in a steel production plant in Brescia, Northern Italy for at least one year. . Individual exposures to ambient PM10 were estimated based on measurements in the 15 work areas and time spent by the study subjects in each area; exposure to metals was evaluated by urinary metal levels. We analysed plasma levels of soluble NOX2-derived peptide (sNOX2-dp), a marker of activation of NOX2 (the catalytic core of NAPDH oxidase) and two validated markers of oxidative stress, namely plasma levels of 8OH-dG and urinary isoprostanes. Workers were exposed to a wide range of PM10 levels (between 44-2270 μ g/m3). The three markers sNOX2-dp, 8OH-dG and urinary isoprostanes showed an high and positive statistically significant correlation (r sNOX2-dp /8OH-dG = 0.73, p < 0001; r sNOX2-dp /urin isoprost= 0.72, p < 0001; r 8OH-dg /urin isoprost= 0.73, p < 0001). sNOX2-dp and urinary isoprostanes were associated with levels of PM10 (sNOX2-dp: βstd=2.48, p=0.05; urinary isoprostanes: βstd=101.24, p=0.02) in multivariable regression models adjusted for age, body mass index and smoking. Metal components of PM10 were not associated with the oxidative markers analysed.
Conclusion: This study demonstrated for the first time a positive association between PM10 and NADPH oxidase activation in a population of highly PM exposed subjects. These findings, suggesting a specific role for PM10 in inducing reactive oxygen species (ROS) formation, deserve further investigation on the role of PM in oxidative stress .
- © 2011 by American Heart Association, Inc.