Abstract 12248: B Lymphocyte Deficiency Attenuates Cold-Induced Elevation of Blood Pressure
Background & Objective. The major function of B lymphocytes is to produce antibodies and cytokines. The purpose of this study was to determine if B cells play a role in the pathogenesis of cold-induced hypertension.
Methods & Results. Two groups of wild type (WT) mice and two groups of B cell-deficient mice were used (all males, 5 mice/group). After blood pressure stabilized and no difference of BP was found between groups, 1 group of WT and 1 group of B cell-deficient mice were exposed to cold intermittently (5°C, 7 hours/day). The remaining groups were kept at room temperature (RT, 25°C) and served as controls. Systolic, diastolic and mean BPs of the WT group began to increase at 9 weeks after intermittent exposure to cold. BP of this group reached a maximal level (mean BP = 140±4 mmHg) by week 14. In contrast, BP of the B cell-deficient group did not increase during intermittent exposure to cold and maintained at the control level (mean BP = 91±3 mmHg, RT-WT). Therefore, B cell deficiency abolished the cold-induced elevation of BP. It is noted that intermittent exposure to cold increased macrophage infiltration, ET1 production, and in situ superoxide production in the kidney of WT mice. Interestingly, B cell deficiency eliminated the cold-induced increases in these parameters.
Conclusion. B lymphocytes may play a critical role in the development of cold-induced hypertension probably via the inflammation-ET1-superoxide pathway.
- © 2011 by American Heart Association, Inc.