Abstract 12126: Rho-Associated Kinase (ROCK) Activity and Left Ventricular Hypertrophy in Patients with Essential Hypertension
Background: In vivo and in vitro experimental studies have shown that Rho-associated kinases (ROCKs) play an important role in Ca2+ sensitization and myocardial hypertrophy. Activation of ROCKs is associated with cardiovascular diseases, including hypertension. Hypertension is associated with left ventricular hypertrophy (LVH). However, there is no evidence for a direct association between ROCK activity and myocardial hypertrophy in humans. The purpose of this study was to determine the relationship between ROCK activity and the degree of LVH in patients with essential hypertension.
Methods and Results: We evaluated ROCK activity in peripheral leukocytes by Western blot analysis in 29 hypertensive patients with LVH (21 men and 8 women; mean age, 62.2±12.5 yr) and 32 hypertensive patients without LVH (16 men and 16 women; mean age, 59.2±15.3 yr). ROCK activity was defined as the ratio of phospho myosin-binding subunit (MBS) on myosin light chain phosphatase to total-MBS. There was no significant differences between other parameters, including systemic and forearm hemodynamics, glucose metabolism and lipid profile, in the two groups. ROCK activity was significantly higher in the LVH group than in the non-LVH group (0.65±0.16 vs. 0.45±0.12; P<0.001). The expression levels of total-MBS, ROCK1 and ROCK2 were similar in the two groups. Univariate analysis showed that activity of ROCKs and systolic blood pressure were correlated with LV mass index (r=0.482, P<0.001 and r=0.296, P=0.021, respectively). Multivariate analysis revealed that ROCK activity and systolic blood pressure were independent risk factors of LVH in patients with hypertension.
Conclusion: These findings suggest that ROCK activity is a reliable predictor of the degree of myocardial hypertrophy in patients with hypertension.
- © 2011 by American Heart Association, Inc.