Abstract 11715: A Brief High-Fat Diet Induces Leukocyte Recruitment to Femoral Artery in Mice with Normal Arterial Shear Flow
[Objective] Though high-fat diet (HFD)-induced metabolic disorders are closely related to atherosclerosis, their causative role in vascular inflammation is not fully understood unless using genetically-modified animals. We thus decided to precisely document the onset of vascular inflammation and atherosclerosis by HFD in wild type C57BL/6 mice (wt) using intravital microscopic analysis (IVM).
[Methods and Results] wt mice (male, 7weeks) were fed HFD (20%fat, 1.25%cholesterol) for 1, 2, 4 and 8 weeks. The interaction of leukocytes and the non-injured femoral artery was monitored by IVM. We discovered leukocyte recruitment as early as 2 weeks after HFD compared to normal chow diet (NC). The leukocyte adhesion was gradually increased at 4 weeks after HFD (HFD, 4.67+/−2.68/104um2 vessel surface; NC, 0.13+/−0.13; p<0.05) and further increased at 8weeks (HFD, 4.88+/−1.72; NC, 0.00+/−0.00; p<0.05). Simultaneous measurement of serum lipid profile revealed significant increase of total cholesterol (HFD, 121.7+/−7.6mg/dl; NC, 66.0+/−1.5, p<0.0001) at 4 weeks after HFD. Serum MCP-1 level was also significantly increased in HFD groups at 4 weeks after feeding HFD (HFD, 268.13+/−41.79pg/ml; NC, 115.14+/−10.43, p<0.05), but not at 2 weeks (HFD, 130.44+/−10.14; NC, 116.34+/−17.42).
[Conclusion] Current data discovered unexpectedly rapid onset of vascular inflammation that initiates atherosclerosis and shed a light on the importance of machinery which translates dietary nutrients to the macrovascular inflammation.
- © 2011 by American Heart Association, Inc.