Abstract 11629: Rho-Associated Kinase (ROCK) Is Activated in Patients with Acute Myocardial Infarction
Background: Rho-associated kinases (ROCKs) play an important role in vascular smooth muscle contraction, endothelial function, and many cellular functions, including cardiomyocytes. However, there is no information on ROCK activity in patients with acute myocardial infarction (AMI). The purpose of this study was to determine changes in ROCK activity during the acute phase of AMI.
Methods and Results: We evaluated leukocyte ROCK activity by Western blot analysis in 39 patients with AMI (33 men and 6 women; mean age, 64±12 yr) and 39 age- and gender-matched control subjects (33 men and 6 women; mean age, 64±13 yr). ROCK activity was defined as the ratio of phospho myosin-binding subunit (MBS) on myosin light chain phosphatase to total MBS. ROCK activity within 2 hours of AMI onset was higher in the AMI group than in the control group (1.13±0.44 vs. 0.69±0.38; P=0.02). ROCK activity promptly increased to 1.34±0.49 after 3 hours and reached a peak of 1.99±0.61 after 1 day (P=0.09, P=0.01 vs. within 2 hours of AMI onset, respectively) and then gradually decreased to 1.68±0.58 after 7 days and to 1.18±0.56 after 14 days (P=0.04, P=0.45 vs. within 2 hours of AMI onset, respectively) The expression of total MBS was similar in the two groups and in any follow-up periods in patients with AMI.
Conclusions: These findings suggest that ROCK activity is dramatically changed after AMI. ROCK activity may be a new biomarker of AMI. Inhibition of ROCK activity may be a therapeutic target for prevention of injury after AMI.
- © 2011 by American Heart Association, Inc.