Abstract 11156: Tissue- and Plasma-Specific MicroRNA Signatures for Atherosclerotic Abdominal Aortic Aneurysm
Background: Atherosclerotic abdominal aortic aneurysm (AAA) is a progressive, gradually developing aortic rupture that results in death in the absence of surgical intervention. Key factors that regulate the initiation and progression of AAA pathology are unknown, making targeted interventions difficult. MicroRNAs play a fundamental role in atherosclerosis, and atherosclerotic coronary artery disease is characterized by tissue- and plasma-specific microRNA signatures. Despite this, little is known about AAA-specific microRNA expression. This study aimed to examine tissue- and plasma- microRNAs signatures exist for AAA.
Methods and Results: AAA and normal wall tissues were sampled from patients undergoing AAA repair (n=10; mean age, 69±6 years) and those undergoing aortic valve replacement surgery (n=5; mean age, 65±4 years), respectively. MicroRNA expression was assessed by high-throughput microRNA arrays, followed by real-time PCR for microRNAs that showed significant differences between the two groups. An apoptosis-related microRNA (miR-15a), inflammation-related microRNAs (miR-124a, miR-146a, miR-155, miR-223), and endothelium-expressed microRNAs (miR-126, let-7f, miR-21) were significantly upregulated in AAA tissues compared to controls. Significant correlations were found between expression of miR-124a, miR-146a, and miR-223 and monocyte chemoattractant protein-1; miR-126 and miR-223 and tumor necrosis factor-α; and miR-146a and transforming growth factor-β. Expression of microRNAs upregulated in AAA tissues was significantly reduced in plasma of patients with AAA (n=21; mean age, 75±8 years) compared to healthy controls (n=6; mean age, 46±10 years).
Conclusions: The expression of some microRNAs was specifically upregulated in AAA tissues, warranting further studies not only on the function of microRNAs in AAA pathogenesis, but also on the possibility to utilize a microRNA signature as a biomarker for AAA diagnosis.
- © 2011 by American Heart Association, Inc.