Abstract 10541: Linkage Between C-Reactive Protein and Triglyceride-Rich Lipoprotein Metabolism
Background: Inflammation plays an important role in atherosclerosis. Elevated C-reactive protein (CRP) levels are associated with a greater risk of cardiovascular disease. Our goals were to study CRP metabolism, and to determine its relationships with lipoprotein metabolism.
Methods: Nine subjects with combined hyperlipidemia (5 men and 4 postmenopausal women) underwent a 15-hour primed-constant infusion with deuterated leucine. CRP was purified from the plasma density fraction greater than 1.21 g/ml by affinity chromatography. Lipoprotein fractions were separated by sequential density ultracentrifugation. Isotopic enrichment was determined by gas chromatography/mass spectrometry. Kinetic parameters were determined using compartmental modeling.
Results: The subjects had mean LDL-C levels of 147.5±25.0 mg/dl and mean CRP levels of 3.4±2.4 mg/L. The mean CRP production rate (PR) was 0.050±0.012 mg/kg/day and the mean CRP fractional catabolic rate (FCR) was 0.343±0.056 pools/day. Men had a higher CRP pool size (PS) and PR than women. (PS in men vs women 16.23±5.72 vs 8.45±2.34 mg, p=0.26; PR 0.056± 0.019 vs 0.040± 0.012 mg/kg/day; p=0.49). CRP PS was significantly correlated to PR (r=0.93; p<0.001), but not FCR. CRP PS was also related to body mass index (r=0.79; p=0.02). There was a significant association between CRP FCR and triglyceride rich lipoprotein (TRL) apoB100 FCR (r=0.74, p=0.04), as well as between CRP PS and TRL apoB48 FCR (r= -0.90, p=0.002), suggesting a relationship between CRP and TRL hepatic metabolism.
Conclusion: The main determinant of plasma CRP levels was CRP production rate; however a linkage between CRP metabolism and TRL apoB100 and apoB48 catabolism was noted.
- © 2011 by American Heart Association, Inc.