Abstract 10354: Dynamic Regulation of Circulating MicroRNA During Acute Exhaustive Exercise and Sustained Aerobic Exercise Training
Background: MicroRNA are intracellular mediators of essential biological functions. Recently, plasma-based “circulating” microRNA (c-miRNA) have also been shown to control cellular processes, but the c-miRNA response to human exercise remains unknown.
Objective: We sought to determine whether c-miRNA are dynamically regulated in response to acute exercise and sustained aerobic training using a longitudinal, repeated measures study.
Results: C-miRNA involved in angiogenesis (miR-20a, miR-210, miR-221, miR-222, miR-328), inflammation (miR-21, miR-146a), skeletal and cardiac muscle contractility (miR-21, miR-133a), and hypoxia/ischemia adaptation (miR-21, miR-146a, and miR-210) were measured at rest and immediately following acute exhaustive cycling exercise in competitive male rowers (n=10, age=19.1 ± 0.6 years) before and after a 90-day period of sustained rowing exercise training. Distinct patterns of c-miRNA response were observed, adhering to 4 major profiles (Figure). 1) C-miRNA (miR-146a and miR-222) that respond to acute exercise before and after sustained training (A, B); 2) C-miRNA (miR-21 and miR-221) that respond to acute exercise before but not after sustained training (C, D); 3) C-miRNA (miR-20a) that respond to sustained training but not acute exercise (E). 4) Nonresponsive c-miRNA (miR-133a, miR-210, miR-328) (F, G, H). A linear correlation was observed when assessing peak exercise levels of miR-146a with Vo2max (r = 0.63, p=0.003), indicating that miR-146a levels reflect peak exercise capacity. A linear correlation was also present between the change (pre-training vs. post-training) in resting miR-20a and the change in Vo2max (r=0.73, p=0.02), suggesting that miR-20a expression reflects training-induced changes in cardiorespiratory fitness.
Conclusions: These results suggest the potential value of c-miRNA as biomarkers and their possible roles as physiological mediators of exercise-induced cardiovascular adaptation.
- © 2011 by American Heart Association, Inc.