Abstract 283: Oxidant and Antioxidant Levels During Brain Hypothermia Therapy After Cardiopulmonary Resuscitation
Oxidative stress has important roles in global brain ischemia. The control of free radical production is one of the targets in the treatment for prevention from brain damage. Therapeutic brain hypothermia therapy (BHT) has neuroprotective effect and is thought to have the effect on the control of free radical production. On the other hand, the kinetic of free radical during BHT remains unclear. The aim of this study is to investigate of the kinetic of oxidative stress and endogenous bio-antioxidant potential during BHT in the patients with post cardiopulmonary resuscitation (post CPR). Twenty one post CPR patients are enrolled. Patients were treated at 33°C. The generation of free radicals was evaluated in each plasma sample by the colorimetric determination of reactive oxygen metabolites (d-ROM test) using the free radical analytical system (FRAS; Health&Diagnostic Limited Co., Italy). The serum antioxidant abilities were measured by the BAP (bioantioxidant potency) test performed with FRAS (Health&Diagnostics Limited Co., Italy) ROM level during hypothermic phase (33°C) was suppressed significantly in comparison to pre-induction phase (333.6±79.0 CarrU vs 266.7±57.6 Carr U, P>0.05). BAP level was also suppressed during hypothermic phase (2523.0±550.5 IU vs. 2021.4±272.2 IU, P>0.05). ROM levels were increased along the rewarming (33–36°C). BAP levels also recovered along the rewarming. Especially, BAP levels were rapidly recovered during 35–36°C. In conclusion, we demonstrated that BHT suppressed free radical production in clinical settings. On the other hand, BHT also led to suppression of bio-antioxidant potential. Our data also indicated that the imbalance of oxidant and antioxidant balance exists in rewarming phase (33–35°C). Supporting therapies of antioxidant potential in rewarming phase in BHT may settle some troubles during rewarming phase in BHT.
- © 2010 by American Heart Association, Inc.