Abstract 216: Effects on Anti-Inflammatory Agent on Neurologic Recovery for Asphyxial Cardiac Arrest in Rats
Background: Rewarming after therapeutic hypothermia for comatose survivor of cardiac arrest, is essential step. However, some studies reported that neurological status was deteriorated during rewarming phase. We hypothesized that the administration of anti-inflammatory agent would reduce the neurological damages.
Objective: We assessed the hypothesis that the administration of anti-inflammatory agent during rewarming phase would reduce the neurological damages in asphyxial cardiac arrest model treated with therapeutic hypothermia.
Methods: Forty adult male Sprague-Dawley rats were assigned at random to 4 groups: normothermia (NT, maintaining a body temperature of 36–38°C); normothermia and administration of dexamethasone during rewarming phase (NT+D); hypothermia (HT, maintaining a body temperature of 30–32°C); hypothermia and administration of dexamethasone during rewarming phase (HT+D). Rats were chemically paralyzed with intravenous vecuronium and the ventilator was disconnected for producing complete cardiac arrest. After 5 minutes without any interventions, the ventilator was reconnected and continuous external chest compressions were performed, at a rate of 200 compressions/min until return of spontaneous circulation was observed. Neurologic deficit score (NDS) was determined after the recovery period at 24, 48, and 72hrs after ROSC. The NDS has a range from 0 (death) to 80 (normal). The NDS examination was performed by a trained examiner blinded to temperature group, and the primary outcome measure of this experiment was defined as the 72-hr NDS.
Results: A total of 40 rats were subjected to cardiac arrest and resuscitation. The baseline weight (mean 348g), duration to cardiac arrest (mean 135 sec), and duration of CPR (mean 34 sec) were similar between groups. All animals survived for the first 24 hrs, and two rats of NT group and one of HT group died at 72hrs. The 72hr-NDS of HT+D group was 79.6, which was significantly higher than that of any other study group (NT+D, 69.9; HT, 65.8; NT, 52.4, respectively).
Conclusions: In conclusion, the administration of dexamethasone during rewarming phase can significantly reduce neurologic damages in this asphyxial cardiac arrest model.
- © 2010 by American Heart Association, Inc.