Abstract 21460: Differences of Diameter of Pulmonary Capillary Vessels in Patients with Pulmonary Hypertension Using Scanning Electron Microscope
Objectives: Idiopathic pulmonary arterial hypertension (IPAH), pulmonary veno-occlusive disease (PVOD), and pulmonary capillary hemangiomatosis (PCH) are included in the same group (group 1) of clinical classification of pulmonary hypertension. Histological changes in the small pulmonary arteries (ie, intimal fibrosis and medial hypertrophy) are similar in these 3 diseases, and clinical presentations of them are often indistinguishable. However, we recently reported differences of three-dimentional structure of pulmonary capillary vessels in these 3 diseases (Miura and Nakamura et al. Circulation 2010). Here, we analyze and compare diameters of pulmonary capillary vessels of them.
Methods and Results: The lungs were obtained from patients with IPAH, PVOD, PCH and control subjects without pulmonary hypertension undergoing lung transplantation or at autopsies. We made microvascular corrosion replicas by perfusion with methacrylate resin (Mercox) from the pulmonary arterial system, and observed them with a scanning electron microscope. Then we measured diameters of pulmonary capillary vessels. The diameters of pulmonary capillary vessels were largest in PVOD (The order of diameter, n = 100 vessels: PVOD (12.550 micrometer) > PCH (11.890 micrometer) > IPAH (10.802 micrometer) > Control (10.172 micrometer)).
Conclusions: The diameters of pulmonary capillary vessels are different between 3 diseases. These findings provide an insight into the basic mechanism responsible for pulmonary hypertension.
- © 2010 by American Heart Association, Inc.