Abstract 20805: Angiogenic and Inflammatory Whole Blood Transcriptional Fingerprint at the Site of Coronary Occlusion in Acute Myocardial Infarction
Background: Recent studies have shown that inflammatory cytokines are elevated at the site of occlusion during myocardial infarction. Our aim was to study the mRNA profile of angiogenic and inflammatory genes in whole blood sampled at the site of the coronary occlusion in patients with STEMI.
Method and results: In 5 consecutive patients with ST-Elevation Myocardial Infarction (STEMI), blood was sampled at the site of occlusion (local) and in the systemic circulation (peripheral) during primary percutaneous coronary intervention. RNA was extracted from whole blood samples. Among 221 genes involved in angiogenesis, inflammation and atherosclerosis, 24 were shown to be modulated using a custom-designed DNA array technology at the site of the coronary occlusion. In 28 STEMI patients, 7 out of these 24 genes were significantly upregulated in local versus peripheral blood at real-time quantitative PCR (all P<0.05). Three genes belonged to the chemokine family (CCL2, CCL18 and CXCL12), 3 genes belonged to cell-cell, and cell-extracellular matrix family (FN1, CDH5 and SPP1) and 1 gene belonged to the lipoprotein family (APOE).
Conclusion: We identified a set of whole blood transcripts induced at the site of the coronary occlusion in the acute phase of myocardial infarction. They point at the predominant role of chemokines, cell-extracellular matrix and lipoprotein alterations in the pathophysiology of acute myocardial infarction and initial response to myocardial injury.
- © 2010 by American Heart Association, Inc.