Abstract 18135: Angiogenesis during Embryonic Development is Dependent on Blood Flow in Zebrafish
Introduction: Embryonic vascular system development is directed or modulated by genetic and epigenetic factors. The latter includes blood flow-induced shear stress and blood pressure-induced circumferential strain. Much has been learned about the genetic mechanisms underlying the development of the vascular system. It is necessary to understand how changes in vessel structure are influenced by hemodynamic factors such as blood flow. Within the circulatory system, blood flow regulates vascular remodeling, stimulates hematopoietic stem cell formation and has an important role in the pathological process of vascular disease. However, few details are known about effect of blood flow on angiogenesis during embryonic development in zebrafish.
Hypothesis: Blood flow may actively contribute to angiogenesis during embryonic development in zebrafish.
Methods: The transgenic zebrafish (flk1: gfp×gata1: desred) was used in this study. There were three experimental groups:zebrafish embryos which were treated by high concentration tricaine had no blood flow(n=60),treated by low concentration tricaine had low blood flow (n=60) and control group with no tricaine (n=60). After treated 10hours (26hpf-36hpf), the heart rate, velocity of blood flow and the development of the caudal vein were determined by using confocal microscopy imaging in live zebrafish embryos. And the zebrafish embryos body length was observed by fluorescence microscope.
Results: It was indicated from imaging and movie that most of caudal vein lacked in high concentration tricaine (52/60), small of caudal vein lacked in low concentration tricaine (54/60) and the caudal vein was intact in control group (58/60). However, there were no significant differences on zebrafish embryos heart rate in the three groups (high: 56.3±2.87/min; low: 57.0±2.91/min; control: 58.5±3.57/min). Our date show that blood flow, but not the heart rate, was essential for caudal vein development during angiogenesis. Interestingly, blood flow regulated vascular development but had no effect on the body length in zebrafish.
Conclusions: These findings demonstrate that blood flow initiated by the embryonic heart contraction stimulate and regulate the embryonic vascular development in zebrafish angiogenesis.
- © 2010 by American Heart Association, Inc.