Abstract 18128: Mesenchymal Stem Cell Seeding Promotes Reendothelialization of the Endovascular Stent
Introduction: Mesenchymal stem cells (MSCs) are nonhematopoietic progenitor cells that are initially present in bone marrow. The bone marrow-derived MSCs have the capability of differentiating in vitro into endothelia, adipocytes, chondrocytes, osteocytes, myocytes, and neurons. It has been confirmed by a lot of researches that MSCs contribute to the repairing of the damage of tunica intima vasorum and plays an important role in re-endothelialization of the impaired intima.
Hypothesis: We assessed the hypothesis that the MSCs seeding stent would been made and it had better potential of re-endothelialization and anti-restenosis than 316L stainless steel stents (316L SS stents) and gluten and polylysine coated stents.
Methods: MSCs were separated from the bone marrow of rabbits by cultivating all marrow cells in the plates, and amplificated and depurated them in vitro. Immunofluorescence and immunocytochemistry were used to identify MSCs with anti-CD29, anti-CD44 and anti-CD34. Ultrasonic atomization spraying was adopted to give the stent a protein coating consisting of glutin and polylysines. Then, cells seeding stents were made according to the optimal rotation culture conditions (planting density: 1×105 cells/ml, rotating time: 6 h, velocity: 0.4 rpm, one rotation) . With the male New Zealand white rabbits were used in the study. 316L SS stents, protein-coated stents and MSCs seeding stents were deployed in the infra-renal abdominal aorta of rabbits to detect the efficiency on promoting re-endothelization by SEM at different time (1 week, 4 weeks, 12 weeks) in vivo.
Results: MSCs of high purity had been obtained, and determined as CD29+CD44+CD34- cells. MSCs were found covering the whole surface of the stent and were in good condition and kept growing.
Results: of the animal experiments showed that the re-endothelialization of the MSCs coated stent had been completed within one month, which considerably inhibited the intimal hyperplasia and in-stent restenosis.
Conclusions: In conclusion, the stent seeded with MSCs promotes re-endothelialization and inhibits the intimal hyperplasia and in-stent restenosis comparing with the 316L SS stents and the gluten and polylysine coated stents.
- © 2010 by American Heart Association, Inc.