Abstract 17609: Early Cardiac Gene Transcript Levels in Peripheral Blood Mononuclear Cells in Patients With Untreated Essential Hypertension
Background: Cardiac hypertrophy and remodelling is accompanied by the activation of the “foetal gene program.” We assessed the expression of early cardiac genes, implicated in the hypertrophic growth response of the adult heart, in peripheral blood mononuclear cells of patients with essential hypertension and its relationship to ambulatory blood pressure monitoring (ABPM) parameters and to echocardiographic left ventricular (LV) mass.
Methods: 24-hour ABPM, echocardiography and blood sampling were obtained in 62 untreated participants with essential hypertension. Blood samples from 38 healthy individuals were included for comparison. Peripheral blood mononuclear cells (PBMCs) were isolated and gene transcript levels were determined by quantitative real time reverse transcription PCR.
Results: Myocardin (3.92±0.68 versus 2.092±0.67, p=0.001), GATA4 (3.48±0.68 versus 0.32±0.08, p<0.001) and Nkx2.5 (208.91±35.01 versus 129.75±49.70, p<0.001), were upregulated in hypertensive patients compare to controls. In hypertensive patients, transcript levels of myocardin (r=0.698, p<0.001) and GATA4 (r=0.374, p=0.003) showed significant positive correlations with 24-hour systolic BP as well with mean BP (r=0.626, p<0.001) and (r=0.340, p=0.007) respectively. A significant positive correlation between myocardin and 24-hour pulse pressure (r=0.467, p<0.001) was also observed. Myocardin (r=−0.606, p<0.001) and GATA4 (r=−0.453, p<0.001) transcript levels also showed significant negative correlations to the mean 24-hour dipping status. Additionally, myocardin (r=0.341, p=0.007), GATA4 (r=0.337, p=0.007) as well as Nkx2.5 (r=0.325, p=0.010) transcript levels showed significant positive correlations with LV mass index.
Conclusions: Myocardin and GATA4 transcript levels correlate significantly with 24-hour ABPM parameters defining them as potential candidate biomarkers in hypertension. Early cardiac gene transcript levels in PBMCs of hypertensive patients associate with LV mass and they may reflect activation of the hypertrophic response gene network in these patients.
- © 2010 by American Heart Association, Inc.