Abstract 17201: Evaluation of a Bioengineered Stent Designed to Capture Circulating Endothelial Progenitor Cells in a Human Ex Vivo Shunt Model
Introduction: The bioengineered Genous stent aims to promote vascular healing by capture and sequestering of circulating endothelial progenitor cells to the surface of the stent struts, resulting in accelerated reendothelialization. Previous studies have suggested that in rabbit and porcine arteries, implantation of the CD34+ antibody coated stent resulted in rapid coverage of the stent by a functional endothelial lining. Here, we assessed the function of the CD34+ capturing stent and bare metal stents, when exposed to the human circulation when implanted in a humanex vivo shunt model.
Methods and Results: 15 Patients undergoing routine coronary angiography received an extracorporeal femoral A-V shunt containing BMS and Genous stents under continuous monitoring of flow. In some bare metal stent after exposure to the circulation, mural thrombi were observed, whereas the CD34+ Genous stents remained patent. Confocal and SEM analysis of the Genous stents showed coverage of the stent struts within 30 min with circular flat cells suggestive of EPC cells. qPCR analysis of eluted cells from the stent surface demonstrated expression of endothelial markers (KDR1(P<0.001), E-Selectin (P<0.045), with a decrease of pro-thrombogenic markers in Genous stent compared to BMS(TFPI (P=0.005), PAI-1 (P<0.05)). For the evaluation of long term endothelialization, New Zealand white rabbits received BMS and Genous stents in the carotid arteries for 7 days (n=8). qPCR analysis identified increased levels of the endothelial markers (Tie1,Tie2,CD34, PECAM, VEGFR1,eNOS) in all carotid arteries treated with Genous versus BMS stents.
Conclusions: We showed that the Genous stent is capable in vivo to efficiently capture EPCs resulting in accelerated endothelialization in the human and rabbit circulation.
- © 2010 by American Heart Association, Inc.