Abstract 16342: First-pass Contrast-enhanced Myocardial Perfusion MR Imaging in Mice on a 3 Tesla Clinical MR Scanner
Introduction: First-pass contrast enhanced myocardial perfusion MR imaging in rodents has so far not been possible due to the challenging temporal and spatial resolution requirements.
Hypothesis: New spatio-temporal undersampling schemes such as k-t SENSE permit the acquisition of first pass myocardial perfusion MR data in rodents on a clinical MR scanner and quantitative estimates of myocardial blood flow.
Methods: We developed a new first pass perfusion MR method for rodent imaging on a clinical 3.0 Tesla scanner (Philips Healthcare, Best, the Netherlands) that employed 10-fold k-space and time domain undersampling with constrained image reconstruction using temporal basis sets (k-t PCA) to achieve a spatial resolution of 0.2 × 0.2 × 1.5mm3 and an acquisition window of 43ms. The method was tested in 5 healthy and 4 infarct mice (C57BL/6J).
Results: Data were acquired successfully in all mice at heart rates of 495.1±45.8bpm. Signal-intensity-time profiles showed a percentage myocardial signal increase of 141.3±38.9% in normal mice, compared with 44.7±32.4% in infarcted segments. Mean myocardial blood flow (MBF) by Fermi-function constrained deconvolution in control mice was 7.3±1.5 ml/g/min, comparable to published literature, with no significant differences between three myocardial segments. In infarcted segments, MBF was significantly reduced to 1.2±0.8 ml/g/min, (p<0.01).
Conclusion: This is the first report of first-pass myocardial perfusion MR in a mouse model. Data were acquired on a 3 Tesla scanner using an approach similar to clinical acquisition protocols, thus facilitating translation of imaging findings between rodent and human studies. Figure legend: A-E: Control mouse. F-J: Infarct model. Images A-D and F-I show the contrast passage through the RV, LV and myocardium. Images E and J are histological cuts showing the anterior infarct in the infarct mouse, matching the perfusion defect on CMR images.
- © 2010 by American Heart Association, Inc.