Abstract 16159: Differential Expression of Epigenetic Modifying Factors and Enzymes Between Induced Pluripotent Cells, Embryonic Stem Cells and Somatic Cells
Due to their therapeutic potential in regenerative medicine, iPS cells have been successfully generated via the reprogramming of somatic cells. However, little is known about the molecular and epigenetic circuitry which initiates and maintains pluripotency and allow for directed differentiation of iPS cells. In addition, the epigenetic differences between iPS cells and ES cells at the molecular level are still to be fully elucidated. Since the remodeling of the epigenetic code during somatic cell dedifferentiation is one of the key modifications that allow a somatic cell to gain its pluripotent identity, a better understanding of these codes is crucial in understanding iPS cells. Mouse iPS cells were generated by exposing reversibly permeabilized mouse fibroblasts (NIH3T3) to mouse ES cell protein extracts. The expression level of selected epigenetic modifying enzymes and epigenetic modifying factors was assayed using Real-Time PCR arrays. The expression levels of selected molecules were verified at both the RNA and protein level. Out of the 84 epigenetic factors assayed, 58% were differentially expressed in the iPS cells as compared to the NIH3T3 as opposed to only 13% when compared to the ES cells. This pattern was similar to that of epigenetic modifying enzymes where out of 84 assayed enzymes 70% were differentially regulated when comparing iPS cells with NIH3T3 as opposed to 25% when comparing iPS to stem cells. Certain key molecules like CDYL, NSD1, CBX7 and HDAC1 showing high degree of up regulation were identified and are being investigated for their function. Analysis and understanding of these epigenetic enzymes and factors and how they are important in defining a cells identity could give us a better understating of how iPS cells gain and maintain their pluripotency and how the epigenetic code could be modified to direct specific differentiation.
- Stem/progenitor cells
- Molecular biology
- Cardiovascular development
- Regenerative medicine stem cells
- © 2010 by American Heart Association, Inc.