Abstract 15752: The Distribution of Circulating microRNA and their Relation to Coronary Disease
MicroRNAs (miRNAs) are small RNAs that play an important role in the negative regulation of gene expression by suppressing protein translation. MicroRNAs have been detected in cell-free plasma and have been related to the presence of prostate cancer. However, the extent of miRNA expression in blood and circulating cells and its relation to cardiovascular disease have only been explored in small studies examining less than a dozen miRNAs. To understand the extent of expression of miRNA in human blood and determine if it is associated with cardiac disease, whole blood, platelets, mononuclear cells, plasma, and serum from normal individuals were screened for all currently measurable (846) miRNAs using high-throughput micro-fluidic quantitative RT- PCR (qRT-PCR). Two-hundred and fifty four, 153, 284, 20, and 34 miRNAs were found to be expressed in moderate to high levels in whole blood, platelets, mononuclear cells, plasma, and serum, respectively. Between whole blood, platelets, PBMCs, plasma, and serum there was significant overlap, although some miRNAs were uniquely expressed. To determine the clinical relevance of these observations, plasma miRNA (n=846) levels were measured in 84 patients presenting for cardiac catheterization. Eight plasma miRNAs were found to have over 2-fold increased expression in patients with significant coronary disease (≥70%) as compared to those with minimal coronary disease or normal coronary arteries. Expression of miR-490-3p, miR-494, and miR-769-3p were found to be significantly different. Using a multivariable regression model including medications and cardiovascular risk factors, hsa-miR-769-3p was found to be significantly correlated with presence of significant coronary atherosclerosis. Using a high-throughput qRT-PCR based method, miRNAs are found to be widely expressed in human blood with differences expressed between fractions. Specific miRNAs from circulating plasma are associated with the presence of coronary disease.
- © 2010 by American Heart Association, Inc.