Abstract 155: Estrogen Reduces Pancreatic Inflammation Following Severe Burns in Rats
Background: Multisystem and remote organ failure following burn injuries remains a significant cause of death each year. The mechanisms relating such organ failure to the burn injury are currently not fully understood. However, it is known that the systemic and remote effects of severe burns are at least in part driven by an increase in inflammation. This post-burn inflammatory response is regulated by certain factors which are released from the burn site; these factors include tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), interleukin 10 (IL-10), and prostaglandins. Previous humans studies have demonstrated that the pancreas and pancreatic function are negatively affected following remote burn injuries. More specifically, beta-cell function and plasma levels of IL-1 have been correlated with patient mortality. In an effort to blunt the inflammatory response following injury, administration of estrogen in animal models has resulted in both decreased levels of inflammatory cytokines statistically and various remote organs, as well as a decrease in mortality. It is therefore hypothesized that administration of estrogen post-burn may protect the pancreas following remote burn injuries.
Purpose: The objective of this study is to elucidate the effect of acute estrogen treatment on IL-1β, IL-6, IL-10 and TNF-α levels in the pancreas following burn injury.
Methods: In this study, male rats received 40% total body surface area burns. Fifteen minutes following burn, the animals received a SQ injection of either placebo (corn oil) or 17 β -estradiol (0.5 mg/kg). The pancreases were harvested at various time points within the proceeding 24 hours, and then cytokine levels were measured.
Results: Administration of estradiol significantly (p < 0.02) decreased the cytokine levels (IL-1b, IL-10, and TNF-a) in the pancreas to a fraction (0.52, 0.39, and 0.33 respectively) of that found in the placebo/burn group at multiple time points within twenty-four hours.
Conclusions: In the pancreas, following remote severe burn injury, estrogen decreases levels of inflammatory cytokines. Results from these studies will help further our understanding of how estrogens may protect the pancreas and encourage systemic stability of the body following burn injuries.
- © 2010 by American Heart Association, Inc.