Abstract 14939: Deposit of Reconstituted High Density Lipoprotein Modulates Neointimal Hyperplasia in Lesions of Balloon-Injured Rat Carotid Artery
Background and aim: High density lipoprotein (HDL) has multiple anti-atherosclerotic effects. We tried to investigate the antirestenosis effects of reconstituted HDL (rHDL) and its mechanism of action in balloon injured rat carotid artery model.
Methods and Results: rHDL was prepared with plasma-derived apoA-I and soybean PC by a molar ratio of 1 to 150. Eighty mg/kg of rHDL was administered through tail vein before surgery, next day, and third day following surgery. Injured carotid arteries were harvested at 2 and 4 weeks after surgery. Intima to media (I/M) ratio by rHDL administration was reduced by 25-fold (p<0.034). After 2 and 4 weeks of injury, strong induction of MMP9, MMP12, PCNA and Ki67 were seen in neointimal lesions. Infiltrations of monocytes and neutrophils were seen in the neointimal area. rHDL selectively inhibited induction of MMP9, and smooth muscle cell proliferation as well as monocytes infiltration in neointima. ApoA1 of human origin was seen to be depositied in the neointima up to 4 weeks. Rats treated with rHDL showed significantly higher expression of heat shock protein 27 (HSP27) in neointima.
Conclusion: rHDL effectively inhibited the neointimal hyperplasia after balloon injury of rat carotid arteries. It inhibits smooth muscle cell proliferation and enhances endothelial cell proliferation, which might be mediated through Hsp27.
- © 2010 by American Heart Association, Inc.