Abstract 13629: MicroRNAs May Control MRNA Networks in Circulating PBMCs During the Acute Phase of Kawasaki Disease
Background: MicroRNAs (miRs) are small noncoding RNAs of 18-25 nucleotides that mediate gene silencing through imperfect hybridization to 3’ untranslated regions (3’UTR) in target mRNAs. MiRs modulate a variety of biological activities including immunological reactions. Though Kawasaki disease (KD) may be associated with immunological problems, the involvement of miRs in KD has not been reported.
Methods and Results: We performed mRNA and miR microarray analysis of peripheral blood mononuclear cells isolated from acute KD patients, who were responsive to intravenous immunoglobulin treatment, or from healthy controls. The data were analyzed using GeneSpring GX 11.0 software and Ingenuity Pathways Analysis tools. Prior to treatment miR-93, miR-150, miR-877 were down-regulated and miR-21, miR-92b, miR-182, miR-296-5p, miR-320d, miR-494, and miR-1826 were up-regulated by comparison with controls. The levels of each of these 10 miRs normalized after treatment. The expression of SGCZ, EIF4G3, C10ORF140, TSHZ3 and ACCN2, which were previously reported to be controlled by these miRs, also normalized upon treatment.
Conculusion: Changes in the expression of these RNAs may contribute to pathogenesis of KD, and provide KD-specific biomarkers that could improve diagnosis and lead to novel therapeutic targets.
- © 2010 by American Heart Association, Inc.