Abstract 12992: Quantitative Coronary Optical Coherence Tomography Analysis for the Automated Evaluation of Thrombus Components and Heterogeneity: Ex-vivo Study
Introduction: Previous studies have demonstrated the efficacy of coronary optical coherence tomography (OCT) for the detection of thrombus. The goal of this study is to investigate the utility of quantitative OCT analysis in evaluating the components and heterogeneity of thrombus with ex-vivo materials.
Methods: We evaluated 16 specimens of coronary artery thrombus obtained by thrombectomy catheter from 9 patients (ST-elevation myocardial infarction: 8, stent thrombosis: 1) who underwent primary percutaneous coronary intervention. The thrombi were immersed in saline immediately after the thrombectomy and OCT image was obtained with time-domain OCT Imaging System (LightLab Imaging, Inc.). Images were acquired during automatic pull back at 1 mm/s and 20 frames/s. Quantitative OCT analysis for all contiguous frames was performed by the dedicated automated software (OCT system software, Light Lab Inc.). Signal attenuation and normalized standard deviation of signal (NSD) was evaluated quantitatively (Figure).All thrombi were stained with hematoxylin and eosin, and divided into three groups; white thrombus: consisting mostly (>67%) of platelets/white blood cells/fibrin, red thrombus: consisting mostly (>67%) of red blood cell, heterogeneous thrombus: other than white and red thrombus.
Results: Totally 629 frames were analyzed. Mean size of thrombus was 0.90±1.29 mm3. Red thrombus showed the highest signal attenuation (white vs. hetero. vs. red: 2.96±2.04 vs. 3.38±0.99 vs. 4.36±0.90, p<0.005), whereas heterogeneous thrombus showed the highest NSD (white vs. hetero. vs. red: 0.179±0.031 vs. 0.189±0.028 vs. 0.167±0.021, p<0.005).
Conclusions: The present ex-vivo study showed the utility of quantitative OCT analysis on the automated evaluation of thrombus components and heterogeneity. Quantitative coronary OCT analysis may contribute to the further interventional innovation by scrutinizing the pathogenesis of acute coronary syndrome.
- © 2010 by American Heart Association, Inc.