Abstract 12408: Weight Loss with Low-Fat Diet Improved Hepatic Inflammation While Weight Loss with Caloric Restriction of High-Fat Diet Improved Adipose Tissue Inflammation in Mice with Diet-Induced Obesity
Obesity is associated with chronic inflammation in adipose tissue (AT) and liver, which is implicated in the development of obesity-related diseases. Diet-induced weight loss is commonly used to treat obesity and effectively reduces risk for obesity-related diseases. However, it is still controversial which type of diet is most effective to treat obesity. Our study tested the hypothesis that weight loss with low-fat diet (LFD) or with restriction of high-fat diet (HFD) has different effects on inflammation in mice with obesity. Weight loss was induced in mice with HFD-induced obesity by either switching from HFD (42% of kcal from fat) to LFD (13% of kcal from fat) and maintaining on LFD ad libitum for 4 weeks or by restricting HFD intake (rHFD) to match the body weight of mice with LFD-induced weight loss. At 4 weeks after switching from HFD to LFD, body weight was reduced by 23±1% (n=16) from baseline. To achieve the same reduced body weight required reduced calorie intake from HFD (daily intake: 9.7±0.4 kcal in rHFD group vs. 12.6±0.6 kcal in LFD group, P<0.01, n=16/group). Weight loss with either LFD or rHFD decreased fat mass and ameliorated liver steatosis. LFD-induced weight loss caused greater decreases in fat mass and hepatic expression of lipogenic genes and greater improvement in liver steatosis than rHFD-induced weight loss. Weight loss with LFD but not rHFD attenuated hepatic inflammation assessed by chemokine and CD11c expression. However, compared to obese controls, weight loss with rHFD significantly reduced chemokine levels and CD11c+ cells in AT, while weight loss with LFD tended to decrease AT chemokine levels and CD11c+ cells. rHFD-induced weight loss tended to reduce AT chemokines and CD11c+ cells more than LFD-induced weight loss. Both LFD- and rHFD-induced weight loss improved glucose clearance in glucose tolerance test and tended to ameliorate insulin resistance. In Conclusions, our study demonstrated differential effects of weight loss with LFD vs. restriction of calorie intake from HFD in obesity treatment. Weight loss with LFD was superior in alleviating hepatic inflammation and steatosis, whereas weight loss with calorie restriction provided greater amelioration of AT inflammation.
- © 2010 by American Heart Association, Inc.