Abstract 12227: Human C-reactive Protein Enhances Thrombus Formation In Transgenic Rabbits
Objective: High plasma levels of C-reactive protein (CRP) are a powerful predictive marker of cardiovascular events. Several lines of evidence suggest that CRP may have prothombogenic effects. However, whether CRP directly participates in the pathogenesis of thrombosis in vivo has not been fully clarified. We tested whether human CRP (hCRP) affects arterial thrombus formation.
Methods and results: In the current study, we used transgenic (Tg) rabbits expressing hCRP (47.15 ± 8.52 mg/L, n=12) and compared their susceptibility to arterial thrombus formation after balloon injury with that of non-Tg rabbits. Tg rabbits showed a 2.5-fold increase in the thrombus size accompanied by a 1.5-fold increase in fibrin content in the thrombi compared with non-Tg rabbits. Furthermore, tissue factor mRNA expression and activity in the neointimal lesions in hCRP-Tg rabbits were significantly increased compared with those of non-Tg rabbits. In addition, hCRP isolated from hCRP-Tg rabbit plasma induced tissue factor mRNA expression and activity in rabbit cultured vascular smooth muscle cells.
Conclusions: These results suggest that high levels of plasma hCRP are not only a marker for cardiovascular events but also promote thrombus formation by enhancing tissue factor expression.
- © 2010 by American Heart Association, Inc.