Abstract 12102: Pharmacologically Induced Hypothermia with Cannabinoid Receptor Agonist Improve the Outcome of Cardiopulmonary Resuscitation
Early application of therapeutic hypothermia and rapid achievement of target cooling temperature are the key factors for improving outcomes following CPR. Current available techniques for inducing hypothermia are either less effective or cumbersome for early application. In this study, we investigated whether hypothermia could be induced pharmacologically following resuscitation with the cannabinoid CB1/CB2 receptor agonist WIN55, 212–2 in a rat model of CPR. Our hypothesis was that when WIN55, 212–2 is administered following resuscitation, it will reduce the body temperature and will therefore improve the outcome of CPR. Ventricular fibrillation (VF) was induced in 10 Sprague-Dawley rats. CPR was initiated after 6 minutes of untreated VF. Defibrillation was attempted after 8 minutes of CPR. Thirty minutes after resuscitation, animals were randomized to receive either WIN55, 212–2 (1.0 mg/kg/hr) or vehicle placebo (1.4 ml/kg/hr) as a control for 6 hours. Blood temperature was decreased from 37°C to 34°C four hours after resuscitation progressively following infusion of WIN55, 212–2. Significantly better post-resuscitation myocardial function and lower NDS (138 ± 106 vs 399 ± 199, p<0.05) were observed in the WIN55, 212–2 treated animals, and this was associated with longer durations of survival (61 ± 15 vs 32 ± 23) compared with the control group. Therapeutic hypothermia following CPR could be induced pharmacologically with a cannabinoid receptor agonist improving post-resuscitation myocardial and cerebral functions, and duration of survival.
- © 2010 by American Heart Association, Inc.