Abstract 11010: Serum Sphingolipids Level as a Novel Potential Indicator for Early Detection of Cardiac Ischemia
Objectives: We tested the hypothesis that sphingolipid concentrations would increase sharply after even transient cardiac ischemia following temporary coronary artery occlusion during percutaneous coronary intervention (PCI) in humans, thereby proving insight into early pathophysiological processes that occur during early cardiac ischemia.
Background: Animal studies suggest that sphingolipids are important signaling molecules produced by cardiac tissue during ischemic stress and are thought to play key roles in ischemic pre and post-conditioning, and pretreatemnt with sphingolipid analogues experimentally protects against ischemia reperfusion injury.
Methods and Results: Venous samples were collected from either the coronary sinus (n=7) or femoral vein (n=24) of 31 patients aged 40–73 years-old at 1, 5 min and 12 h, following elective PCI. Plasma sphingolipids levels were assessed by HPLC. At 1 min coronary sinus levels of sphingosine 1-phosphate (S1P), sphingosine (SPH) and sphinganine (SA) were increased by 314%;, 115%; and 614%;, respectively (n=7), while peripheral blood levels increased by 79%;, 68%; and 272%; (n=24). By 5 min, coronary sinus S1P and SPH levels increased further (720%;, 117%;), as did peripheral levels of S1P alone (792%;). Where troponin T was detectable at 12 h (10 of 31), a strong correlation was found with peak S1P (R2=0.818; P<0.0001).
Conclusion: This study, for the first time, demonstrates the behaviour of plasma sphingolipids following transient cardiac ischemia in humans with dramatic increases in S1P, SPH and SA and significant correlation with 12 h troponin levels. These observations may support the important role of sphingolipids in pre and post conditioning mechanisms occurring in humans and may have the potential to become novel, robust predictors of transient or prolonged ischemia.
- © 2010 by American Heart Association, Inc.