Abstract 3692: Alteration of Parasympathetic/Sympathetic Ratio in the Infarct Myocardium After Schwann Cell Transplantation Modified Electrophysiological Function of Heart: A Novel Antiarrhythmic Therapy
Background: Neural remodeling after myocardial infarction (MI) could cause fatal ventricular arrhythmia (VA). Schwann Cells (SCs), which is important for neurogenesis, lost dramatically post MI. We investigated feasibility to modify nerve system regeneration after MI and efficacy to prevent ventricular arrhythmia by SC transplantation.
Methods and Results: Immediately after creation of MI, syngenic Lewis rats were randomized into cell transplantation (n=80) and control groups (n=72). SCs were isolated from sciatic nerves and 5×106 cells were intramyocardially injected into the infarcted region. We found that expressions of nerve growth factor, vascular endothelial growth factor, growth associated protein 43(GAP43), connexin 43, laminin in myocardium in SC group were significantly higher than those in control at 7 and 14 days after cell transplantation. Immunohistochemical staining illustrated that sympathetic and parasympathetic nerves increased in both groups, but SCs transplantation significantly increased parasympathetic/sympathetic ratio at 14 days after cell injection(figure a & b⇓). Programmed electrical stimulation (PES) and echocardiography were also performed at 1, 7, and 14 days post implantation. The SCs significantly decreased the occurrence rates of PES-induced VA by 3-fold at 2 weeks after cell injection. However, SCs did not restore the damaged heart function,
Conclusions: Transplanted SCs in the infarct myocaridun secreted multiple biological molecules, which alter ratio of parasympathetic/sympathetic never density to prevent PES-induced VA. SCs transplantation might be a novel cell-based antiarrhythmic therapy after MI.