Abstract 3540: Impaired the Mobilization and Functional Activity of Bone Marrow Derived Circulating Progenitor Cells by Elevated CRP Levels During Acute Phase Myocardial Infarction
Background: Bone marrow-derived circulating progenitor cells (BM-CPCs) are mobilized in adult peripheral blood (PB) during the acute myocardial infarction(AMI) period and may contribute to the regeneration of infarcted myocardium. The purpose of the present study is to determine whether the mobilization as well as functional activity of BM-CPCs in PB depends on infarct associated inflammatory markers following AMI.
Methods: Mobilization of CD34/45 + and CD133/45 + cells by flow cytometry (n = 100) and the functional activity of BM-CPCs by migration assay (n = 44) were measured in patients with AMI and in healthy controls (n = 20). Global ejection fraction (EF) and the size of infarct area were determined by left ventriculography.
Results: Mobilization of CD34/45 + and CD133/45 + BM-CPCs significantly increased, peaking on day 7 and decreased on day 8 after AMI as compared to the healthy controls. Additionally, the migratory capacity of BM-CPCs improved significant on day 7 as compared to on day 1 after AMI (VEGF: p<0.001, SDF-1: p = 0.002). The mobilization of BM-CPCs on day 1 after AMI showed significantly negative correlation with CRP levels on day 1 after AMI (CD34/45+: p<0.001 r = −0.58; CD133/45 +: p =0.003 r = −0.43), the number of CVRFs (CD34/45+: p<0.001 r = −0.50, CD133/45+: p=0.02 r = −0.33), infarct size (CD34/45+: p<0.001 r = −0.81; CD133/45 + p<0.001 r = −0.54). Likewise, the migration capacity of BM-CPCs on day 1 after AMI showed significantly negative correlation with CRP levels, CVRFs and infarct size. The elevated CRP and leukocytes levels on day 1 after AMI were significant associated with decreased global EF as well as increased infarct size.
Conclusions: On the basis these findings, it is tempting to speculate that this improved spontaneous mobilization and functional activity of BM-CPCs is a response to myocardial repair after AMI and this maximum effect of BM-CPCs mobilization may achieve on day 7 after AMI. Moreover, The elevation of these inflammatory markers such as CRP seems to be an independent predictor for reduced mobilization and functional activity of BM-CPCs during acute phase of myocardial infarction and thereby may decrease myocardial repair after AMI