Abstract 3406: Completely Autologous Valved-Conduit With the Sinus of Valsalva (BIOVALVEs) Constructed by in vivo-Tissue Engineering: Successful Pulmonary Valve Replacement in Beagle Model
Objectives: As a practical concept of regenerative medicine, we address constructing autologous prosthetic substitutes by simple and safe in vivo tissue engineering without any use of special clean facilities nor complicated in vitro cell processing. In this study, we firstly developed autologous valved conduits (BIOVALVEs) with the sinus of valsalva by this unique techinique, which were successfully implanted as pulmonary valves in the beagle model.
Methods and Results Mold for BIOVALVE organization was assembled with specially designed convex- and concave-shaped silicone rods with a small aperture of three-leaflet shape between them. The concave-shaped rod had three projections resembling the sinus of Valsalva. The molds were placed in the dorsal subcutaneous spaces of beagle dogs. After 4 weeks of implantation, autologous connective tissues completely covered the surface of the molds and migrated into their apertures. BIOVALVEs with three leaflets inside of the conduit with the sinus of Vasalva consisting only of autologous tissues were obtained after removing the molds. They had appropriate compliance and robustness (>3000 mmHg) as the conduit-valves for aortic full root replacement. Tight valvular coaptation and sufficient open orifice area were observed in vitro. The BIOVALVEs were implated to the main pulmonary arteries as allogeneic conduit-valves under CPB. Postoperative UCG demonstrated smooth movement of the leaflets with little regurgitation at least for 4 weeks.
Conclusion: Completely autologous valved conduits (BIOVALVEs) with the sinus of valsalva were firstly developed by utilizing the living body as a reactor for tissue organization.