Abstract 3255: Myoblast Sheet Implantation Can Prevent the Impairment of Diastolic Function and Late Remodeling After Left Ventricular Restoration in Severe Ischemic Cardiomyopathy
Objectives Impairment of diastolic function and late remodeling are concerns after left ventricular restoration (LVR) for ischemic cardiomyopathy. This study aims to evaluate the effects of combined surgery of myoblast sheets (MS) implantation and LVR.
Methods and Results Rat myocardial infarction model was established 2 weeks after left anterior descending artery ligation. They were divided into 3 groups: sham operation (n=8; Group Sham), LVR by plicating the infracted area (n=8; Group LVR) and MS implantation with LVR (n=8; Group MS+LVR). Echocardiographic study revealed significant improvement of LV function after the operation in Group LVR and in Group MS+LVR (Fig 1-A, B⇓). However, significant LV re-dilatation and decrease of ejection fraction were observed in Group LVR 4 weeks after the operation. MS implantation combined with LVR prevented those later deteriorations of LV function in Group MS+LVR. A hemodynamic assessment using a pressure-volume loop showed significantly preserved systolic and diastolic function in Group MS+LVR (Table 1⇓). Histological examination revealed cell hypertrophy and LV fibrosis were significantly less in group MS+LVR than in the other 2 groups.
Conclusions MS implantation attenuated the impairment of diastolic function and the late remodeling after LVR, suggesting that MS implantation may improve long term outcome of LVR for ischemic heart disease.