Abstract 2462: Combining Xenon and Mild Therapeutic Hypothermia to Reduce Neurological Sequelae After Cardiac Arrest
Introduction: Combining xenon (Xe) and mild therapeutic hypothermia (MTH) provides greater protection against hypoxic/ischemic neurological injury in rodents than MTH alone.
Hypothesis: Xe and MTH will also significantly improve neurological injury in a porcine model of cardiac arrest (CA) compared to MTH alone.
Methods: 14 Pigs were subjected to 10 minutes of CA before 6 minutes of cardiopulmonary resuscitation was initiated and defibrillation attempted. Animals were randomized to receive either 16h of MTH plus 1h of ventilation with 70% Xe started one hour after successful resuscitation (MTH + Xe), 16h of MTH alone (MTH) or 16h of anesthesia under normothermic conditions (NT). After rewarming and weaning from the ventilator, animals were assessed for neurological outcome on five postoperative days using a neurological deficit score (NDS), ranging from 0 (normal cerebral performance) to 100 (brain death).
Results: All surviving animals showed severe neurological deficits at postoperative day 1 (Fig. 1⇓). While MTH + Xe animals significantly improved their performance during the observation period from day 1 to 5 (52±24 vs. 5±6 p =0.02) pigs from the other groups did not. Animals receiving MTH + Xe had a significant better NDS at day 5 as compared to animals in the NT group (5±6 vs. 53±40 p =0.03).
Conclusion: In this CA animal model Xe in combination with MTH provided significant greater neuroprotection than MTH alone.