Abstract 1627: Doppler Fourier Domain Optical Coherence Tomography: A Novel Technique to Analyze Exercise-dependent Changes in Vascular Dynamics of Wild-type and LDL Receptor Knockout Mice in vivo
Regular exercise is beneficial for the prevention of endothelial dysfunction and atherosclerosis. However, the measurement of changes in vascular dynamics in murine models in vivo is difficult. Therefore, we developed a novel Doppler FD OCT device allowing determination of blood flow in murine arteries and veins with a spectrum read out rate of 12 kHz. The high temporal resolution of the system can be used to quantify the pulse from real-time cross-sectional images by analyzing the intensity alternation caused by changes from systole to diastole inside the vessel lumen. For visualizing of flowing blood in the saphenous artery or vein, flow velocities were colour-coded on the structural images. Tail cuff measurements confirmed the heart rate obtained by FD OCT at systolic blood pressure. Distribution of visceral fat tissue and musculature was analysed by peripheral quantification computer tomography (pQCT) using XCT-Research SA bone scanner. We analyzed murine vascular dynamics of small arteries using in vivo Doppler Fourier Domain optical coherence tomography (FD OCT) in LDLR knockout compared to C57BL/6 wild-type mice kept under sedentary conditions or with access to voluntary wheel running. Vascular function of murine saphenous arteries of male C57BL/6 and LDLR knockout mice was analyzed at 23±2 weeks of age after feeding a diet containing 5.1% soy bean fat and no cholesterol. The results are summarized in the table⇓ (mean±SD). In conclusion, using this novel Doppler FD OCT technique we were able to image the blood flow velocity, the flow rate during the systolic and diastolic phase of the cardiac cycle. We could show beneficial effects of voluntary running on vascular function of small arteries of wild-type and LDL receptor knockout mice in vivo.