Abstract P153: Curcuma and Curcumin in Experimental Cardiac Arrest in Rats
Purpose of the study: Due to positive effects of curcuma and curcumin in models of focal cerebral ischemia and neurodegenerative diseases, we investigated their effects on the neurological outcome in our model of cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) in rats.
Materials and methods: After approval of the animal care committee, 76 male wistar rats were subjected to a 6 min CA. The animals were assigned to the following 4 groups:
Curcumin intraperitoneal (300 mg/kg, 5 min after restoration of spontaneous circulation (ROSC); n=12),
Curcumin (feeding for 28 d before CA, 2g/kg/d; n=14)
Curcuma (feeding for 28 d before CA, 2g/kg/d; n=11).
Sham operated animals served as non ischemic controls (n=12). All animals were kept normothermic for the first 24 h after ROSC. The neurological outcome was examined using the Morris-water-maze-test (MWM) for 7 days before CA and on 3d and 7d post ROSC. Inflammatory response was analysed before CA and 6h after ROSC using ELISA testing for IL-1, IL-6, IL-10 and TNF. After 7 days the neuronal damage in the hippocampal CA-1 sector was assessed using TUNEL- and Cresylviolet-staining.
Results: At 3d after ROSC all animals showed a significant neurological deficit when compared to pre-CA (p≤0.01). All treatment groups, but not the placebo group (p=0,012 7d after ROSC vs. pre-CA values), reached the pre-CA performance on 7d after ROSC in the MWM. In between the groups no significant difference could be shown. Neither in the histology nor in the ELISA testing significant differences were observed between the groups.
Conclusions: Applying the MWM in this model of global cerebral ischemia after CA, a significant neurological deficit is evident 7d after CA in the placebo group but not in the treatment groups indicating a better recovery in these groups. However no significant effect of the treatment is evident when using intergroup comparison. Despite recent studies, showing clear beneficial effects on the neurological outcome in models of focal cerebral ischemia, no benefit for the neurological outcome after global cerebral ischemia could be achieved from the application of Curcumin or Curcuma.